Skip to main content

Table 1 Effect of PhaR based protein purification method for purifying EGFP, MBP and LacZ, respectively

From: Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification

 

Yield of purified protein

LacZ activity (Units/mg a)

Target proteins

mg/g nanoparticles

mg/g wet weight of bacteria

Pre-induced whole cell lysate

Induced whole cell lysate

Purified LacZ

EGFP

9.14 ± 1.2

4.39 ± 0.5

   

MBP

8.17 ± 1.06

4.01 ± 0.3

   

LacZ

6.55 ± 0.9

3.16 ± 0.3

7.22 ± 0.9

167 ± 7.5

486.77 ± 15

  1. a One unit of β-galactosidase activity hydrolyzes 1.0 μmol ONPG to o-nitrophenol and D-galactose per minute at 37°C.