Figure 8
From: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
Fermentation studies of GFP expression. (A) Growth profiles of pBAD24M-GFP, SDM I and SDM II at 30°C in DH5α cells in a bioreactor. The arrow indicates the point of induction. The growth was monitored at A600 nm and DO levels were indicative of effective viable cells. (B) Fluorescence of the GFP samples of harvest samples of pBAD24M-GFPuv (lane 1), SDM I (lane 2) and SDM II (lane 3) under uv light after loading on SDS-PAGE without sample boiling. Arrow indicates the fluorescent protein.