Figure 7
From: Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
Western blot of GFP expressed from WT and SDM II clones. M: Medium molecular weight marker 97-14 kDa); lane 1: Supernatant of WT GFP expressed from JM 109; lane 2: Supernatant of GFP SDM II expressed from JM 109 cells; lanes 3 and 4: WT GFP and SDM II mutant expressed in BL21 cells respectively. The upper arrow shows the intact GFP (29 kDa) while the lower arrow shows the degraded form of GFP (of < 14 kDa) observed only with WT GFP clone and negligible with the GFP SDM II clone.