Table 4 Codon usage table designed for high level expression during methanol induction in P. pastoris.
From: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
Amino acid | Codon | Frequency [%] | Amino acid | Codon | Frequency [%] |
|---|---|---|---|---|---|
A | GCU | 54.3 | P | CCU | 42.3 |
| Â | GCC | 23.3 | Â | CCC | 6.0 |
| Â | GCA | 20.5 | Â | CCA | 52.0 |
| Â | GCG | 2.0 | Â | CCG | 0.0 |
C | UGU | 70 | Q | CAA | 66.5 |
| Â | UGC | 30.0 | Â | CAG | 33.5 |
D | GAU | 33.5 | R | CGU | 20.2 |
| Â | GAC | 66.5 | Â | CGC | 0.0 |
E | GAA | 47.0 | Â | CGA | 1.2 |
| Â | GAG | 53.0 | Â | CGG | 1.2 |
F | UUU | 31.0 | Â | AGA | 71.5 |
| Â | UUC | 69.0 | Â | AGG | 6.0 |
G | GGU | 64.3 | S | UCU | 48.2 |
| Â | GGC | 8.5 | Â | UCC | 27.2 |
| Â | GGA | 25.3 | Â | UCA | 11.3 |
| Â | GGG | 2.3 | Â | UCG | 4.3 |
H | CAU | 20.0 | Â | AGU | 4.3 |
| Â | CAC | 80.0 | Â | AGC | 4.3 |
I | AUU | 52.0 | T | ACU | 46.5 |
| Â | AUC | 43.7 | Â | ACC | 38.0 |
| Â | AUA | 4.3 | Â | ACA | 9.5 |
K | AAA | 25.5 | Â | ACG | 6.0 |
| Â | AAG | 74.5 | V | GUU | 48.5 |
L | UUA | 11.5 | Â | GUC | 29.8 |
| Â | UUG | 43.7 | Â | GUA | 6.5 |
| Â | CUU | 21.2 | Â | GUG | 15.3 |
| Â | CUC | 7.7 | W | UGG | 100 |
| Â | CUA | 2.5 | Y | UAU | 22.0 |
| Â | CUG | 13.5 | Â | UAC | 78.0 |
M | AUG | 100 | Stop | UAA | 75.0 |
N | AAU | 21.5 | Â | UAG | 0.0 |
| Â | AAC | 78.5 | Â | UGA | 25.0 |