From: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
 |  | Accessible activity after permeabilization | |||
---|---|---|---|---|---|
Isopropanol concentration | Incubation time | Tv1a_mc | Tv1_mc | ||
[%, by vol.] | [h] | [U/g wcw] | η | [U/g wcw] | η |
- | - | 6 | ~0.01 | 5 | <0.01 |
0 | 0.3 | 100 | 0.20 | 321 | 0.23 |
4 | 0.3 | 118 | 0.23 | Â | Â |
8 | 0.3 | 141 | 0.28 | Â | Â |
10 | 0.0 | 100 | 0.20 | Â | Â |
10 | 0.3 | 184 | 0.36 | 430 | 0.33 |
10 | 0.7 | 193 | 0.38 | Â | Â |
10 | 1.5 | 206 | 0.41 | Â | Â |
10 | 2.5 | Â | Â | 579 | 0.45 |
10 | 3.0 | 226 | 0.45 | Â | Â |
10 | 5.3 | Â | Â | 627 | 0.49 |
10 | 10.7 | Â | Â | 621 | 0.48 |
12 | 0.3 | 176 | 0.35 | 424 | 0.33 |
15 | 0.3 | 170 | 0.34 | Â | Â |
20 | 0.3 | 64 | 0.13 | Â | Â |
40 | 0.3 | 14 | 0.03 | Â | Â |