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Table 2 Comparison of relevant host strains used for production of Tv DAO.

From: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Expression host

Activity

Reference

Trigonopsis variabilis I

4,620 U/LIV

 

Pichia pastoris

7,596 U/LV

[16]

Pichia pastoris

23,000 U/L IV

[17]

Pichia pastoris

12,532 U/LIV

[22]

Pichia pastoris II

218,926 U/LV

this study

Saccharomyces cerevisiae

~110 U/g dcwV

[14]

Kluveromyces lactis

~150 U/g dcwV

[14]

Escherichia coli III

12,340 U/LV

[13]

  1. I Induced with N-carbamoyl-D-alanine
  2. II Induced with reduced methanol feed
  3. III Additional D-methionine induction was reported to yield in a 4.5 fold expression improvement
  4. IV Activity measured with D-alanine as substrate
  5. V Activity measured with D-methionine as substrate
  6. dcw dryed cell weight
  7. Non standardized conditions used for assaying the enzyme activity in different publications make direct and quantitative comparison of published data difficult.
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Microbial Cell Factories

ISSN: 1475-2859

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