Figure 3
From: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
Effect of biomass concentration on permeabilization of recombinant Pichia pastoris cells expressing Tv DAO. Cells of Tv1_mc cultivated in a bioreactor were treated with isopropanol for 20 min and 20 h. The activity of permeabilized cells is given as a percentage of the total intracellular activity (1283 U g/wcw) measured after cell lysis. Activity measurements were performed three times resulting in standard deviations of < 5%.