Figure 2
From: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
High-cell-density bioreactor cultivation of P. pastoris strain Tv1_mc using induction with a mix-feed strategy. Values are referenced to oxidase activity obtained using the standard methanol feed and set as 100%. The fed-batch induction phase lasted 90 h in each case. MeOH: 3 mL/h methanol. MeOH/2: 1.5 mL/h methanol, Glu/MeOH: 3 mL/h glucose-methanol mixture, Gly/MeOH: 3 mL/h glycerol-methanol mixture. Activity measurements were performed three times, resulting in standard deviations of < 14%.