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Table 3 Real-time PCR validation of stress-related and TCA cycle genes

From: Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production

 

scFvD1.3 vs Skp/scFvD1.3

scFvD1.3 vs FkpA/scFvD1.3

Gene

Real-time PCR expression ratio

clpS

1.6

1.9

dnaJ

2.1

3.0

fkpB

1.6

1.7

grpE

1.8

1.4

hslR

2.2

2.5

ibpA

-1.1

1.4

ybbN

2.5

2.4

sdhB

-2.1

-2.7

sdhD

-2.5

-2.6

sucD

-2.5

-2.4

  1. Quantitative real-time gene expression comparison of the wildtype scFvD1.3 over the chaperone co-expressing Skp/scFvD1.3 or FkpA/scFvD1.3 cells. Seven upregulated stress-related genes and three down-regulated TCA cycle genes from the microarray were validated with real-time PCR.