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Table 1 Co-expression of Skp and FkpA on scFvD1.3 yield and viability

From: Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production

  scFvD1.3 yield scFvD1.3 cell viability
Protein(s)
co-expressed
(mg/L) Fold increase (%) Fold increase
LacZ (control) 5.4 ± 1.5 - 5.3 ± 4.2 -
Skp 51.2 ± 6.9 10× 46.0 ± 2.8
FkpA 42.6 ± 6.1 45.3 ± 7.6
Skp + FkpA 18.3 ± 2.7 18.0 ± 2.8
  1. While the co-expression of Skp or FkpA alone at 0.01% arabinose resulted in a 8-10 fold increase in scfvD1.3 yield and cell viability over the LacZ control, the co-expression of Skp and FkpA together led to only a 3 fold increase.