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Table 1 Co-expression of Skp and FkpA on scFvD1.3 yield and viability

From: Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production

 

scFvD1.3 yield

scFvD1.3 cell viability

Protein(s)

co-expressed

(mg/L)

Fold increase

(%)

Fold increase

LacZ (control)

5.4 ± 1.5

-

5.3 ± 4.2

-

Skp

51.2 ± 6.9

10×

46.0 ± 2.8

9×

FkpA

42.6 ± 6.1

8×

45.3 ± 7.6

9×

Skp + FkpA

18.3 ± 2.7

3×

18.0 ± 2.8

3×

  1. While the co-expression of Skp or FkpA alone at 0.01% arabinose resulted in a 8-10 fold increase in scfvD1.3 yield and cell viability over the LacZ control, the co-expression of Skp and FkpA together led to only a 3 fold increase.
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Microbial Cell Factories

ISSN: 1475-2859

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