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Table 2 Aromatic metabolites production and yields determined for strain PB12 and SA-producing derivatives.

From: Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

Strain

SA

(g/L)

SA yield

(mol SA/mol glc)

DAHP

(g/L)

DHS

(g/L)

GA

(g/L)

TACY1

(mol aromatic compounds/mol glc)

PB12

ND

---

0.044 ± 0.07

ND

ND

0.00

PB12.SA11

2.82 ± 0.01

0.11 ± 0.00

1.71 ± 0.07

2.79 ± 0.21

0.21 ± 0.06

0.28

PB12.SA21

5.07 ± 0.00

0.21 ± 0.00

0.52 ± 0.00

2.49 ± 0.06*

0.14 ± 0.00

0.33*

PB12.SA22

7.05 ± 0.06

0.29 ± 0.00

0.81 ± 0.04

1.46 ± 0.14

0.08 ± 0.01

0.37*

PB12.SA31

4.35 ± 0.57

0.22 ± 0.04

3.03 ± 0.00

2.12 ± 0.02

0.23 ± 0.04

0.50

PB12.SA41

1.00 ± 0.36

0.03 ± 0.02

0.14 ± 0.00

0.79 ± 0.01

ND

0.07

  1. Values are the average of two independent experiments. 1TACY, Total aromatic compound yield (combined DAHP, DHS, SA and GA molar yields); ND, Non-detected. Mean values within each column with the same superscript (*) (P < 0.05) do not differ significantly with respect to the immediate parental strain (see Methods).