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Table 1 Growth kinetic parameters for strain PB12 and SA-producing derivatives.

From: Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

Strain/derivative

μ a

(h-1)

qs b

(millimol glc g DW-1 h-1)c

PB12

0.48 ± 0.02

5.17 × 10-6 ± 4.07 × 10-7

PB12.SA11

0.41 ± 0.00

2.03 × 10-6 ± 4.79 × 10-7

PB12.SA21

0.42 ± 0.02*

2.5 × 10-6 ± 8.55 × 10 × 10-7*

PB12.SA22

0.42 ± 0.01*

1.93 × 10-6 ± 5.9 × 10 × 10-7*

PB12.SA31

0.32 ± 0.02

7.76 × 10-6 ± 9.67 × 10-8

PB12.SA41

0.45 ± 0.03

2.58 × 10-6 ± 5.39 × 10-7

  1. Values are the average of two independent experiments. a μ, specific growth rate; bqs, specific glucose consumption rate; cDW, dry cell weight. Mean values within each column with the same superscript (*) (P < 0.05) do not differ significantly with respect to the immediate parental strain (see Methods).