Skip to main content
Figure 1 | Microbial Cell Factories

Figure 1

From: Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

Figure 1

Central carbon metabolism and shikimic acid pathways in E. coli PB12 strain lacking the PTS. Glucose transport and phosphorylation are performed by GalP and Glk, respectively [27]. Abbreviations: Glc, glucose; GalP, galactose permease; Glc-6-P, glucose-6-P; Glk, glucokinase; PEP, phosphoenol pyruvate; PYR, pyruvate; Ac-CoA, acetyl coenzyme-A; TCA, tricarboxylic acid cycle; OAA, oxaloacetate; PPP, pentose phosphate pathway; E4P, erythrose-4-P; DAHP, 3-deoxy-D-arabino heptulosonate-7-P; DHQ, 3-dehydroquinic acid; DHS, 3-dehydroshikimic acid; SA, shikimic acid; S3P, shikimate-3-P; EPSP, 5-enolpyruvylshikimate-3-phosphate; CHA, chorismic acid; QA, quinic acid; PCA, protocatehuic acid; GA, gallic acid. Genes and coded enzymes: tktA, transketolase I;pykF, pyruvate kinase I;pykA, pyruvate kinase II;ppsA, phosphoenolpyruvate synthase; aroF, aroG, aroH, DAHP synthase isoenzymes F, G and H, respectively; aroB, DHQ synthase; aroD, DHQ dehydratase; aroE, shikimate dehydrogenase; aroK, shikimate kinase I; aroL, shikimate kinase II; aroA, EPSP synthase; aroC, chorismate synthase; aroZ, dehydroshikimate dehydratase; pobA, p- hydroxy-benzoate hydroxylase [32]. Continuous arrows represent unique reactions catalyzed by one or more enzymes; dotted lines or arrows represent two or more enzymatic reactions or incomplete characterized reactions.

Back to article page