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Table 3 Kinetic parameters of the purified mannan endo-1,4-β-mannosidase

From: Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from Bacillus licheniformis in Escherichia coli

Substrate

Vmax

(μmol min-1mg-1)

Km

(mg ml-1)

k cat

(s-1)

k cat /Km

(mg-1s-1ml)

Glucomannan

30,400

14.9

21,000

1,410

LBG(low viscosity)

45,300

17.5

31,200

1,790

β-D-Mannan

26,400

15.2

18,200

1,200