Figure 4

Effect of pH on the activity (A) and stability (B, C) of B. licheniformis mannan endo-1,4-β-mannosidase. The optimal pH was determined at 50°C using 0.5% LBG in 50 mM of different buffers (A). The pH stability was determined by measuring the remaining activity after incubation at various pH values at 50°C for 30 min (B) and 24 h (C). The buffers used were acetate buffer (black square) from pH 2 - 6; potassium phosphate buffer; KPP (black circle) from pH 6 - 9; and glycine buffer (black triangle) from pH 9 - 12.