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Figure 3 | Microbial Cell Factories

Figure 3

From: Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from Bacillus licheniformis in Escherichia coli

Figure 3

Coomassie staining and zymogram analysis of purified recombinant mannan endo-1,4-β-mannosidases from B. licheniformis. SDS-PAGE analysis of purified recombinant β-mannanase is shown in panel A. M, marker; C, control E. coli lysate; I, input (crude extract); Ft, flow through; w1-3, wash 1-3; e1-2, enzyme from 1st and 2nd elution. Panel B illustrates zymogram analysis of the purified enzyme. Only 1/1000 of the amount used in the left panel was loaded onto the gel. White bands indicate mannan endo-1,4-β-mannosidase activity.

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