Table 3 Oligonucleotides list
Name | Sequence (5'-3') | Restriction site |
|---|---|---|
cotB-up | AAGCTT ACGGATTAGGCCGTTTGTC | Hind III |
cotB-dn | GATATC GGATGATTGATCATCTGAAG | Eco RV |
cotC-up | ACCCAAGCTT TGTAGGATAAATCGTTTG | Hind III |
cotC-dn | GATATC GTAGTGTTTTTTATGCTT | Eco RV |
cotG-up | CCCGAATTC CGAGAAAAAATCC | Eco RI |
cotG-dn | CTTGGATCC TTTGTATTTCTTTTTGACTAC | Bam HI |
ureA-up | GAGGGATCC ATGAAACTCACCCCAAAAG | Bam HI |
ureA-dn | CGCGAGCTC TAGGGCCATACATAGAAAC | Sac I |
ureA1-up | GAAGCGGATATC GGTAAAAAGACTGCG | Eco RV |
ureA1-dn | GGGCCATACACTAGT ACATATTCTTTTCTGCTAATC | Spe I |
hisureA-up | CTCGGTACC TTCTTTTCTGCTAATCTTTTTTTC | Kpn I |
hisureA-dn | TATGCTAG CATGCATCATCACCATCACCATCACTCGAAACTCACCCCAAAAGAG | Nhe I |