Figure 6From: Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis sporesDot blot performed with the indicated concentrations of coat proteins (in μg) extracted from spores carrying Fusion 1 (A, lane 3), Fusion 2 (B, lanes 3-5) and Fusion 3 (C, lane 3) and from wild type spores (lane 2 in all panels). In panel B, lanes 3, 4 and 5 contain proteins of strains KH10 (PY79 carrying cotC::ureA1), KH11 (PY79 cotC::spc cotC::ureA1) and KH12 (PY79 cotC::spc cotU::erm cotC::ureA1), respectively. Purified UreA (in ng, lane 1 in all panels) was also utilized.Back to article page