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Table 1 Percentage of solubilization of IBs in different solvents

From: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin

 

% solubilized protein

Solubilization solution

ferm-flavoHb

flask-flavoHb

0.5% Triton X-100 in 40 mM Tris/HCl, pH 8.0

8 ± 0.6

10 ± 0.9

5% DMSO in 40 mM Tris/HCl, pH 8.0

9 ± 0.9

5 ± 0.4

5% di n-propanol in 40 mM Tris/HCl, pH 8.0

6 ± 0.7

5 ± 0.1

0.2% N-lauroyl sarcosine in 40 mM Tris/HCl, pH 8.0

95 ± 1.0

57 ± 0.8

1% Na-deoxycholate in 40 mM Tris/HCl, pH 8.0

22 ± 1.5

12 ± 0.3

  1. Percentage of solubilization of IBs in different solvents was calculated using as 100% the protein concentration obtained dissolving IBs in urea 8 M and comparing this value with the protein concentration of samples obtained by treatment with different solvents. Evaluation of protein concentration was obtained by measuring the absorbance at 280 nm (Abs280).