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Table 1 Percentage of solubilization of IBs in different solvents

From: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin

  % solubilized protein
Solubilization solution ferm-flavoHb flask-flavoHb
0.5% Triton X-100 in 40 mM Tris/HCl, pH 8.0 8 ± 0.6 10 ± 0.9
5% DMSO in 40 mM Tris/HCl, pH 8.0 9 ± 0.9 5 ± 0.4
5% di n-propanol in 40 mM Tris/HCl, pH 8.0 6 ± 0.7 5 ± 0.1
0.2% N-lauroyl sarcosine in 40 mM Tris/HCl, pH 8.0 95 ± 1.0 57 ± 0.8
1% Na-deoxycholate in 40 mM Tris/HCl, pH 8.0 22 ± 1.5 12 ± 0.3
  1. Percentage of solubilization of IBs in different solvents was calculated using as 100% the protein concentration obtained dissolving IBs in urea 8 M and comparing this value with the protein concentration of samples obtained by treatment with different solvents. Evaluation of protein concentration was obtained by measuring the absorbance at 280 nm (Abs280).