Table 1 Summary of reported yields obtained with the λpL/pR/cI857 thermoinduced expression system.

Recombinant protein localized in cytoplasm

UVRA

pGHY5003

BC; 30 to 42°C

7% TCP

λpl, cI857

IFN-β

pPLc245-HFIF25

BC; 28 to 42°C

4% TCP

λpL/cI857

GXP

pHEGPT

BC, SF; 30 to 42°C

5% TCP

λpL/cI857

IHF α, β

pPlhima-1, pPLhipohim A-s

BC; 31 to 42°C

3 mg/L

λpL/cI857

rpGH

p-LARpGH

BC, SF; 30 to 42°C (3-4 h), temp reached in 5-8 min

15% TCP

λpL/lcI857

rpGH

p-LARpGH

FB; 30 to 42°C

20% TCP

λpL/lcI857

β-gal

pJLACZ

CC; 28 to 40°C

3,037 U/ml/OD

λpL cI857

β-gal

pJLACZ

CC; 28 to 42°C

7,327 U/ml/OD

λpL cI857

SpA-β-gal

BC; 30 to 40°C

6.2 g/L

λpR

SpA-β-gal

FB; 30 to 40°C

19.2 g/L

λpR

β-gal

pACYC177; pRSET-

lacZ

BC, SF; 30 to 42°C (2 min), reducing to 37°C

23500 U

λpL/cI857

β-gal

pACYC177; pRSET-

lacZ

BC, SF- FB; 30 to 42°C (2 min), reducing to 37°C

23000 U

λpL/cI857

β-gal

pACYC177; pRSET-

lacZ

FB; 30 to 42°C (2 min), reducing to 37°C

285000 U

(0.95 g/L)

λpL/cI857

Carbamoylase

pTAH10;

pT-GroE

BC, SF; 30 to 39°C reached abruptly (10 to 20 min), decrease to 37°C

7 U

(0.14 U/mL)

λpLpR, cI857-T7 RNA pol

Carbamoylase

pTAH10;

pT-GroE

BC; 30-39°C (10 to 20 min), decrease to 37°C

1830 U

(1.2 U/mL)

λpLpR, cI857-T7 RNA pol

Carbamoylase

pTAH10;

pT-GroE

FB; 30 to 39°C (10 min), decrease to 37°C

14256 U

(5.8 U/mL)

λpLpR, cI857-T7 RNA pol

TNF-α

pCY-TNF

BC, SF; 30 to 42°C

12% soluble TCP

λpRpL

cI 857

TNF-α

pCY-TNF

BC, 30 to 42°C (6 h)

11% soluble TCP

λpRPL

cI 857

TRAIL

pBV-Trail

FB; 30 to 42°C (4 h)

1.4 g/L

Temperature inducible

GFP

pND-GFP

BC; 30 to 42°C (1 h),

reducing to 30°C

30 mg/L

λpL/cI857

GFP

pND-GFP

BC; 37 to 42°C (1 h),

reducing to 37°C

50 mg/L

λpL/cI857

GFP

pND-GFP

BC, SF; 37 to 42°C (1 h),

reducing to 37°C

7 mg/L

λpL/cI857

GFP

pND-GFP

BC, SF; 37 to 42°C (1 h), reduction to 37°C (1 h), increasing to 42°C (1 h), reducing to 37°C

68 mg/L

λpL/cI857

GFP

pND-GFP

BC, SF; 30 to 42°C (1 h), reduction to 30°C (1 h),

increase to 42°C (1 h),

decrease to 30°C

45 mg/L

λpL/cI857

GFP

pND-GFP

FB; 30 to 42°C (30 min), decrease to 30°C,

increase to 42°C (30 min), decrease to 30°C

273 mg/L

λpL/cI857

IB localized in cytoplasm

IFN-γ

pPL-l

BC, SF; 28°C-42°C

0.3 g/L

λpL/cI857

IFN-γ

pPL-l

FB; 28 to 42°C

7.43 g/L

λpL/cI857

Insulin A- chain-Mut3sY

PMYW-A

BC, SF: BL21; 30 to 42°C

30% TCP

λpl/cI857

Insulin A- chain-Mut3sY

PMYW-A

FB, HCDC; 30 to 42°C

13% TCP

λpl, lcI857

Insulin B- chain- Mut3sY

PMYW-B

FB, HCDC; 30 to 42°C

18% TCP

(4.6 g/L)

λpl, lcI857

IFN-α

pMYW-a

FB; 30 to 42°C

4 g/L

λpl, lcI857

rhBMP-2

pCYTEXP3-BMP-2

BC, SF 30 to 42°C

25% TCP

λpL, cI857

rhBMP-2

pCYTEXP3-BMP-2

FB; 30 to 42°C

20% TCP

(8.5 g/L)

λpL, cI857

hFGF-2

plFGFB

FB; 30 to 42°C (6-8 h)

5-6 g/L

λpRPL cI857

hGH

pET21-hgh

FB (complex media);

30 to 42°C (30 min),

reduction to 37°C (4 h)

11% TCP

(2.0 g/L)

pET21-hgh; pGP1-2 gene 1 of T7 under λpL

hGH

pET21-hgh

FB (glycerol);

30 to 42°C (30 min),

reduction to 37°C (4 h)

15% TCP

(2.7 g/L)

pET21-hgh; pGP1-2 gene 1 of T7 under λpL

IFN-α 2b

pRSET-INFα 2b; pGP1-2

BC, SF; 30 to 42°C

(5, 10 and 15 min)

650 mg/L

λPL, T7 RNA pol, cI857

His-tag-hPPI

λ-PL-cI857 pUC

FB; 30 to 42°C

heat rate 0.4°C/min

3.3 g/L

λpL/cI857

His-tag- hPPI

λ-PL-cI857 pUC

FB; 30 to 42°C

heat rate 0.8°C/min

2.2 g/L

λpL/cI857

His tag- hPPI

λ-PL-cI857 pUC

FB; 30 to 42°C

heat rate 1.7°C/min

1.8 g/L

λpL/cI857

His tag- hPPI

λ-PL-cI857 pUC

FB; 30 to 42°C

heat rate 6°C/min

1.9 g/L

λpL/cI857

Recombinant protein localized in cytoplasm and supernatant

TK

pHETK2

BC, SF; 30°C to 42°C (16 h)

4% soluble TCP

λpL/cI857

TK

pHETK2

BC, SF; 30°C to 42°C (16 h)

4% soluble TCP

λpL/cI857

β-gal

pRA-A1Its-187Z

BC, SF; 30 to 40°C (5 h)

30% TCP

(2.0 g/L)

λpR, cI857, PA1

β-gal

pRA-A1Its-187Z

BC; 30 to 40°C

22% TCP

(2.0 g/L)

λpR, cI857, PA1

scFv

pCMT2b-scFv;pRcd1

BC, SF; 30°C to 42°C (7 h)

37 mg/L

λpL/cI857; Rcd lPR

scFv

pCMT2b-scFv;pRcd1

FB; 30 to 42°C (8 h)

160 mg/L

λpL/cI857; Rcd IPR

Recombinant protein localized in supernatant

Glucagon-SEAP

pBLGlu-2

BC, SF; 30 to 40°C

3.4 mg/L/OD600

λpL/cI857

Recombinant protein localized in periplasm

hGH-fusion to DsbA

DsbA-hGH

BC, SF; 30 to 42°C

19%

λpL/cI857

hGH

lPL-DsbA-hGH/pRK248cIts

FB; 30 to 42°C

heat rate 6°C/min

95 mg/L

λpL -cI857