Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

Table 1 HRPL selected mutants generated by IvAM

Mutant	Amino acid Substitution	Nucleotide change	Mutation type	Location	Secondary structure motif	Distance to the T1 Site (Å)	Distance to the T2/T3 (Å)
6C8	N23K	AAC 69AAA	Tv	Pro-leader
	G62G	₆GGG 186GGA₁₁	Ts	Pro-leader
5H12	V80L	G TA238C TA	Tv	Pro-leader
	S244S	₈TCG 1005TCC₁₄	Tv	Mature protein
10B1	A13V	GC A38GT A	Ts	Pre-leader
	I33T	AT T98AC T	Ts	Pro-leader
	A185A	₂₁GCT 828GCC₁₃	Ts	Mature protein
16B10	A361T	G CG1354A CG	Ts	Mature protein	loop	23	21
	P395P	₇CCC 1458CCT₁₃	Ts	Mature protein
	S482L	TC G1718TT G	Ts	Mature protein	sheet	34	24

Synonymous mutations are underlined; nucleotide change is highlighted in bold; subscript numbers on codons indicate codon usage. Tv, transversions; Ts, transitions.

ISSN: 1475-2859