for VP parent types and different mutants of the evolutionary process. White squares, 24E10 mutant; black triangles, R4 mutant; black squares, 10C3 mutant (best parent type from 3rd generation); white circles, best parent type from 2nd generation; white triangles, best parent type from 1st generation; black circles, native VP expressed in E. coli after in vitro refolded from inclusion bodies. B) T50 for HRPL parent types and mutants of the in vitro evolution. Black circle, 1D11 mutant (4th generation); white triangle, 11A2 mutant (4th generation); black triangle, 7H2 mutant (5th generation); white circle, 6C8 mutant (6th generation); white square, 5H12 mutant (6th generation); black down triangle, 10B1 mutant (6th generation); black square, 16B10 mutant (6th generation). Each point, including the standard deviation, comes from three independent experiments.