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Figure 4 | Microbial Cell Factories

Figure 4

From: Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

Figure 4

directed evolution landscapes. A) Activities of clones from the library of VP mutants prepared by mutagenic StEP + in vivo DNA shuffling, plotted in descending order. B) Initial activities vs thermostabilities in VP mutants. Red dashed lines represent VP-10C3 parent type. Mutants selected for further re-screens are squared (see Material & Methods section for details). C) Activities of clones from the library of HRPL mutants prepared by IvAM, plotted in descending order. D) Initial activities vs thermostabilities in HRPL mutants. Red dashed lines represent 7H2 parent type. Mutants selected for further re-screens are squared.

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