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Table 2 Physiological parameters for BP10001 under different fermentation conditions

From: Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization

 

Xylose Batch

Glucose Fed-Batch

Glucose/Xylose Fed-Batch

qglucose [g/g CDW/h]a

 

0.79

0.65

qxylose [g/g CDW/h]a

0.14

 

0.30 - 0.19c

Yethanol [g/g]b

0.33

0.39

0.38

Yxylitol [g/g]b

0.17

 

0.05/0.19d

Yglycerol [g/g]b

0.038

0.070

0.029

Yacetate [g/g]b

0.023

<0.001

<0.001

C-recovery [%]

99

100

94

  1. a) Uptake rates were determined from data acquired in the first 20 h. S.D.s on uptake rates were < 10%.
  2. b) Yield coefficients (g/g sugar consumed) were calculated using data after a reaction time of 120 h (batch) or 20 h (fed batch). S.D.s were < 10% (except Yglycerol: < 20%). Yacetate in fed-batch reactions was too low for an S.D. to be determined.
  3. c) qxylose decreased over the initial 20 hours of fermentation (see also Figure 5B).
  4. d) Yield coefficients are based on either the sum of consumed glucose and xylose or on consumed xylose alone.
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Microbial Cell Factories

ISSN: 1475-2859

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