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Table 2 Effect of the overexpression of HXT 1 or HXT 7 genes in the S. cerevisiae GRF18U or CEN.PK strains

From: Effect of HXT 1 and HXT 7 hexose transporter overexpression on wild-type and lactic acid producing Saccharomyces cerevisiae cells

Strains Glc
%
YNB% aa mg/L Agitation μ_exp phase
[1/h]
Rate Glc Consumption* [g/L/h] Rate EtOH Production* [g/L/h] Rate LA Production* [g/L/h] Y_EtOH**
[g EtOH/g Glc]
Y_LA**
[g L.A./g Glc]
CEN.PK   2.39 1.34 50 YES 0.28
+/- 0.05
-0.77
+/- 0.08
0.23
+/- 0.09
- 0.19
+/- 0.11
-
CEN.PK [HXT 1] 2.39 1.34 50 YES 0.29
+/- 0.07
-0.83
+/- 0.08
0.27
+/- 0.04
- 0.21
+/- 0.05
-
CEN.PK [HXT 7] 2.39 1.34 50 YES 0.29
+/- 0.02
-0.82
+/- 0.08
0.28
+/- 0.03
- 0.20
+/- 0.03
-
CEN.PK   5.68 2.68 150 YES 0.33
+/- 0.05
-1.66
+/- 0.06
0.42
+/- 0.01
- 0.19
+/- 0.00
-
CEN.PK [HXT 1] 5.68 2.68 150 YES 0.33
+/- 0.03
-1.74
+/- 0.09
0.44
+/- 0.01
- 0.20
+/- 0.01
-
GRF18U   2.39 1.34 50 YES 0.25
+/- 0.05
-0.69
+/- 0.04
0.27
+/- 0.05
- 0.23
+/- 0.01
-
GRF18U [HXT 1] 2.39 1.34 50 YES 0.25
+/- 0.03
-0.79
+/- 0.08
0.32
+/- 0.06
- 0.25
+/- 0.08
-
GRF18U [HXT 7] 2.39 1.34 50 YES 0.25
+/- 0.04
-0.80
+/- 0.07
0.31
+/- 0.06
- 0.24
+/- 0.06
-
CEN.PK [LDH] 2.04 1.34 50 YES 0.12
+/- 0.04
-0.38
+/- 0.07
0.09
+/- 0.08
0.08
+/- 0.09
0.17
+/- 0.01
0.21
+/- 0.01
CEN.PK [HXT 1] [LDH] 2.04 1.34 50 YES 0.14
+/- 0.08
-0.40
+/- 0.05
0.10
+/- 0.04
0.09
+/- 0.06
0.19
+/- 0.09
0.24
+/- 0.01
CEN.PK m850 [LDH] 9.14 1.70 - YES 0.02
+/- 0.00
-1.21
+/- 0.00
- 0.77
+/- 0.00
- 0.67
+/- 0.00
CEN.PK m850 [HXT 1] [LDH] 8.96 1.70 - YES 0.03
+/- 0.09
-1.22
+/- 0.08
- 0.85
+/- 0.02
- 0.76
+/- 0.02
CEN.PK   2.13 1.34 150 NO 0.26
+/- 0.03
-1.22
+/- 0.03
0.41
+/- 0.08
- 0.40
+/- 0.03
-
CEN.PK [HXT 1] 2.13 1.34 150 NO 0.26
+/- 0.04
-1.30
+/- 0.02
0.45
+/- 0.02
- 0.45
+/- 0.08
-
CEN.PK   5.68 2.68 150 NO 0.23
+/- 0.01
-4.30
+/- 0.06
1.37
+/- 0.05
- 0.31
+/- 0.08
-
CEN.PK [HXT 1] 5.68 2.68 150 NO 0.23
+/- 0.04
-4.36
+/- 0.07
1.58
+/- 0.06
- 0.36
+/- 0.09
-
  1. Table summarizes all the data obtained with wild type and engineered yeasts (see first column) grown in the indicated conditions (see columns 2-5). The specific growth, glucose consumption, ethanol and lactate production rates together with yield for ethanol and lactic acid production are given. For the described determinations, the respective standard error is indicated. Glc: glucose. aa: aminoacids. LA: lactic acid. * determined in exponential phase. ** determined at the timing of the highest metabolite (ethanol or lactic acid) production.