Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli

Table 1 Important parameters and results of 6 different batch fermentations using the pET21d⁺/POx expression system.

fermentation	1	2	3	4	5	6
time of induction [h]	0	0	0	6 (OD₆₀₀ = 0.7)	0	0
temp. [°C]	37	30	25	25	22	18
DCW [g/L]	4.5	4.6	4.6	3.2	5.7	4.1
Yx/s [g/g]	0.46	0.46	0.47	0.33	0.56	0.41
μ_max [h^-1]	1.15	0.88	0.48	1.25	0.23	0.20
vol. activity [U/L]	1	45	916	425	1155	1300
process time [h]	25	25	26	22	40	50
productivity [U/L·h]	0	2	32	18.5	28	26
space time yield [mg/L·h]	0	0.3	4.0	2.3	3.5	3.3

ISSN: 1475-2859