Figure 6

SDS-PAGE analysis of IB formation during fermentations at different temperatures and different times of induction using the pCOLD III/POx expression system. After lysis, cell debris containing IB was collected by centrifugation; 100 mg cell debris were washed, resuspended in Laemmli Buffer, heated at 95°C for 5 min and 10 μl aliquots were loaded per lane. Lane 1, 25°C and induction at OD₆₀₀ 0.5; Lane 2, 25°C and induction at OD₆₀₀ 2.5; Lane 3, 20°C and induction at OD₆₀₀ 0.5; Lane 4, 20°C and induction at OD₆₀₀ 2.5; Lane 5, 15°C and induction at OD₆₀₀ 0.5; Lane 6, 15°C and induction at OD₆₀₀ 2.5.