Figure 5
From: Biosynthesis of 2-hydroxyisobutyric acid (2-HIBA) from renewable carbon
Connection of PHB biosynthesis steps to the proposed whole-cell production of 2-HIBA employing the bioisomerization route of aerobic MTBE degradation. In the absence of PHB polymerase activity, a biological system having the ability for overflow metabolism will accumulate 3-hydroxybutyryl-CoA from any suitable carbon source. This common metabolite will be converted to 2-hydroxyisobutyryl-CoA which is hydrolyzed and excreted.