Table 1 16S rRNA gene OTUs in the groundwater bacterial community 15 days after lactate amendment
OTUa | N. of clones | Accession Number of identified OTUs | Closest database hit | Nt identity and Reference | Phylum | Copies 16S rRNA geneb | Normalized % over total clonesc | Characteristics of the closest described relative | Source |
|---|---|---|---|---|---|---|---|---|---|
1** | 26 | FM205003 | Desulfitobacterium dichloroeliminans [AJ565938] | 956/978 (97%) | Firmicutes | 6 | 26.6 - 38.2 | 1,2 DCA dechlorinating bacterium | [37] |
2** | 26 | FM204994 | Dehalobacter sp. WL [DQ250129] | 1480/1489 (99%) | Firmicutes | 5 - 12 | 19.1 - 32.0 | Chlorinated ethanes and 1,2 DCA dechlorinating bacterium | [35] |
3** | 17 | FM205004 | D. metallireducens [AF297871] | 1408/1459 (96%) | Firmicutes | 6 | 17.4 - 25.0 | Couples growth to the reduction of metals and humic acids as well as chlorinated compounds | [36] |
4 | 5 | FM205000 | Uncult Clostridiaceae [AF255644] | 1393/1448 (96%) | Firmicutes | 5 - 12 | 3.7 - 6.1 | Fermentative obligate anaerobe isolated from black mud | [49] |
5* | 2 | FM204997 | Trichlorobacter thiogenes [AF223382] | 1466/1484 (98%) | δ-Proteobacteria | 4 | 3.1 - 4.4 | Reductive dehalogenating bacteria | [50] |
6* | 3 | FM204996 | Uncult. Clostridium [AB186865] | 1413/1426 (99%) | Firmicutes | 5 - 12 | 2.2 - 3.7 | Acetogenic bacterium from acidic sediments | Drake et al., (Unpublished) |
7* | 3 | FM205001 | Clostridium sp. CYP5 [DQ479415] | 1430/1432 (99%) | Firmicutes | 5 - 12 | 2.2 - 3.7 | sulfur-reducing anaerobe isolated from an olive mill wastewater contaminated by phosphogypse | Ben Dhia et al., (Unpublished) |
8 | 2 | FM204995 | Uncult Clostridiaceae [AF255644] | 1347/1444 (93%) | Firmicutes | 5 - 12 | 1.5 - 2.5 | Fermentative obligate anaerobe isolated from black mud | [49] |
9 | 1 | FM204999 | Uncult. Bacteroidetes [AJ488070] | 1446/1450 (99%) | Bacteroidetes | 5 - 6 | 1.2 - 1.5 | Propionate-producing bacterium isolated from plant residue in irrigated rice-field | [51] |
10 | 1 | FM204998 | Clostridium saccharolyticum [Y18185] | 1414/1460 (96%) | Firmicutes | 5 - 12 | 0.7 - 1.2 | Bacteria able to convert a number of mono and disaccharides to ethanol, acetic acid, lactic acid, hydrogen and carbon dioxide | [52] |
11 | 1 | FM205002 | Uncult clone PL-5B10 [AY570634] | 1404/1460 (96%) | Firmicutes | 5 - 12 | 0.7 - 1.2 | Thiosulfate-reducing bacterium from an oil-producing well | [53] |
12* | 1 | FM205005 | Uncult Clostridiaceae [AJ009473] | 1359/1447 (93%) | Bacteroidetes | 5 - 12 | 0.7 - 1.2 | Obligate anaerobic mesophile bacterium isolated from a UASB reactor | Song and Dong (Unpublished) |