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Figure 4 | Microbial Cell Factories

Figure 4

From: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

Figure 4

A schematic picture of the EnBase® Flo cultivation principle. Phase 1 = controlled cultivation in mineral salt medium with a low concentration of complex additives (peptones, yeast extract). Phase 2 = induction with IPTG and addition of complex nutrients (booster mixture). Phase 2a of increased growth rate is followed by Phase 2b of low growth rate and slow production of soluble protein.

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Microbial Cell Factories

ISSN: 1475-2859

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