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Figure 2 | Microbial Cell Factories

Figure 2

From: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

Figure 2

Recombinant protein production in 24 DWPs using different cultivation media. A) Stereoselective R-alcohol dehydrogenase (ADH) from Lactobacillus produced by E. coli RB791 [pQE30:Adh]; B) Protein disulphide isomerase A-domain (PDI) produced by E. coli BL21(DE3)pLysS [pET23:pdi]; C) Multifunctional enzyme type 2 of Drosophila produced by E. coli BL21(DE3) [pPal7:Dm_MFE-2]. In each sub-picture (A, B, C) soluble proteins are shown on the left and total proteins on the right. The upper and lower parts of the pictures present proteins after 6 h and 24 h induction, respectively. To facilitate direct comparison between different cultivation media, all samples were diluted to equal cell concentration before cell lysis and loading on gel. EB = EnBase Flo medium, MSM = Mineral Salt Medium, LB = Luria Bertani, TB = Terrific Broth. Target protein is indicated by arrows.

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Microbial Cell Factories

ISSN: 1475-2859

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