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Figure 1 | Microbial Cell Factories

Figure 1

From: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

Figure 1

Characteristics of recombinant E. coli strain RB791 [pQE30:Adh] cultivations in different culture media. EB = EnBase Flo medium, MSM = mineral salt medium, LB = Luria-Bertani medium, TB = Terrific Broth medium. A: OD600 in 50 ml cultures in 500 ml shake flasks; B) pH in 50 ml cultures in 500 ml shake flasks; C) OD600 in 3 ml cultures in 24-deep well plates. The time point of induction (0.4 mM IPTG) is indicated with a dashed vertical line. EnBase Flo cultures were also supplemented with "medium booster" (complex additives) at the same time with induction. EnBase Flo cultures were grown for 19 h before induction, whereas cultures in MSM, LB and TB media were grown for 2 h before induction. All cultures were run until 24 h after induction.

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Microbial Cell Factories

ISSN: 1475-2859

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