Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1

Table 7 List of strains, constructed plasmids, and primers used in this study

Material	Relevant features	Source of reference
Strains
L. lactis; NZ9000	MG1363 pepN:nisRK, Cloning host	[44]
L. plantarum WCFS1	Cloning host, genomic DNA isolation	[17]
L. plantarum NZ7100	WCFS1 :nisRK, Cloning host	[46]
Plasmids
pNZ7021	Cm^R, pNZ8148 derivative, nisin promoter replaced by pepN promoter	[1]
pNZ7026	Cm^R, pNZ7021 derivative containing the folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1	[18]
pNZ8148	Cm^R, nisin regulated promotor	[44]
pNZ7030	CmR, pNZ8148 derivative containing folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1 in the sense orientation	(this study)
pNZ7031	CmR, pNZ8148 derivative containing folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1 in the antisense orientation	(this study)
Primers
LpfBnco-F	CTGGGATACCCATGG GCATGATTC
LpfPkpn-R	CGTCAAAAGGTACC GGACTAATCATTATTCG
pNis-F	TAGTCTTATAACTATACTGAC
LpfB-R	CTTGCCATTCGGCGTCCCCTCCACCTCAATTTCC
LpfBatg-F	ATGGGCATGATTCGAATTAATAATTTACG
LpfP-xbatest	GAATTTAATTATTTGCGACGCCCAAT
FQPCRfolBS	CCTATCGAAACCAAGGTTCAACA
RQPCRfolBS	ACAAATTCATCGACCACGTTACG
FQPCRfolBAS	TCAACTTGTATGAATGGGTCGTTACA
RQPCRfolBAS	CGTTCACGAGACCATCAATTACG
FQPCRFPS	CATTATTAACGATGTGAACGCCTTT
RQPCRFPS	CGCGACTGTCAGCCATCAAT
FQPCRfPAS	CTAACAGCGTAATCAATTGCTTGGT
RQPCRfPAS	CTTAAGGGTGGCCGGATCA
groES-fo(2)	CCCAAAGCGGTAAGGTTGTT
groES-re(2)	CTTCACGCTGGGGTCAACTT
pfk-fo1	TCCAGGGACGATCGATAATGA
pfk-re1	GCTTGCACGTTGGTGTTGAAC

ISSN: 1475-2859