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Table 5 Relative expression of folB and folP in L. plantarum harboring pNZ8148, pNZ7030, and pNZ7030 after 20 minutes and 4 hours following nisin induction and in the uninduced cultures

From: Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1

   0 ng/ml nisin 25 ng/ml nisin
time minutes L. plantarum harboring Average expression folB - folP Average expression folB - folP
20 pNZ8148 sense 1 1
20 pNZ7030 sense 64 584
20 pNZ7031 antisense 84 2864
240 pNZ8148 sense 1 1
240 pNZ7030 sense 1 38
240 pNZ7031 antisense 3 11
  1. Expression values of the two folate genes, folB and folP, are normalized to groES, and are indicated as average expression folB-folP.