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Table 5 Relative expression of folB and folP in L. plantarum harboring pNZ8148, pNZ7030, and pNZ7030 after 20 minutes and 4 hours following nisin induction and in the uninduced cultures

From: Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1

  

0 ng/ml nisin

25 ng/ml nisin

time minutes

L. plantarum harboring

Average expression folB - folP

Average expression folB - folP

20

pNZ8148 sense

1

1

20

pNZ7030 sense

64

584

20

pNZ7031 antisense

84

2864

240

pNZ8148 sense

1

1

240

pNZ7030 sense

1

38

240

pNZ7031 antisense

3

11

  1. Expression values of the two folate genes, folB and folP, are normalized to groES, and are indicated as average expression folB-folP.