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Table 2 Intracellular and extracellular concentration of 6-hydroxymethylpterin and folate in L. plantarum harboring pNZ7021 and pNZ7026 in the presence and absence of pABA

From: Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1

  

6-Hydroxymethylpterin (nmol/50-ml culture)

Folate μg/L per OD600 unit

L. plantarum harboring

μmax h-1

Intracellular

Extracellular

Intracellular

Extracellular

pNZ7021

0.61 (0.02)

0.1

NDa

ND

ND

pNZ7021 + p ABA

0.60 (0.02)

0.1

NDa

3.93 (1)

8.56 (3)

pNZ7026

0.45 0.02)

3.0

1692

ND

ND

pNZ7026 + p ABA

0.44 (0.01)

0.2

217

216 (29)

3020 (202)

  1. a ND, not detectable
  2. Note: The standard deviation of the folate assay is shown between brackets.