Figure 2

Sequence Analysis. Sequence of the promoter/operator region, identical in pMPAG6 (lacI^q+) and pMPAG77 (lacI^q-) (top line), and alignment with the deletions (bottom line) isolated from lacI^q-E. coli strains harboring the respective lacI^q-expression vector: identical sequences were found in at least 6 independent clones of each construct protein. The -35 and -10 regions are indicated by yellow boxes; the two lac O1 operator sites are marked by red letters (length defined as the LacI contact residues seen by NMR [36], with each symmetry center in bold). The ribosomal binding site (RBS) is highlighted in green and the start codon in light blue, respectively.