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Table 2 Plasmids and corresponding expression products

From: A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

Vectorsa

Gene cloned

Resulting vectorsa

Expression product

pET30a

Ecotin-Strep

pET30a Ecotin-Strep

Ecotin-Strep

pET30a

Ecotin-Ubiquitin-Strep

pET30a Eco-Ubi-Strep

Ecotin-Ubiquitin-Strep

pET30a lacUV5p

Ecotin-Ubiquitin-Strep

pET30a lacUV5p Ecotin-Ubiquitin-Strep

Ecotin-Ubiquitin-Strep

pET30a lacUV5p Ecotin-Ubiquitin-Strep

FLS

pET30a lacUV5p Ecotin-Ubiquitin-FLS

Ecotin-Ubiquitin-FLS

  1. a plasmids with the lacUV5 promoter are named pET30a lacUV5p