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Table 1 Oligonucleotides used in this study

From: A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

Primersa Sequences (5'-3')
NdeI Ecotin F GATATACATATGAAGACCATTCTACCTGCAGTA
Ecotin-Strep SalI R TATAGTCGACTTATTTTTCGAACTGCGGGTGGCTCCAGCGAACTACCGCGTTGTCAAT
Ecotin-Ubi (AS2–7) R CAGGGTCTTCACGAAGATCTGGCGAACTACCGCGTTGTCAAT
Ubi (AS2–7) F CAGATCTTCGTGAAGACCCTGACC
Ubi-Strep XhoI R TATACTCGAGTTATTTTTCGAACTGCGGGTGGCTCCAGCCACCCCTCAGACGGAGGAC
lacUV5p SphI F CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTT
lacUV5p XbaI R CTAGAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGCATG
Ubi-FLS XhoI 1 F TATGAGTCGACCCTGCACCTGGTCCTCCGTCTCCGCGGTG
Ubi-FLS XhoI 2 R CCGCCACCTTTATCGTCATCATCTTTATAGTCACCACCGCGGAGACGGAG
Ubi-FLS XhoI 3 F ATGACGATAAAGGTGGCGGAGGTAGCGGTGGAGGAGGCAGCTGGAGCCAT
Ubi-FLS XhoI 4 R TATACTCGAGTTATTTTTCAAACTGCGGATGGCTCCAGCTGCCTC
  1. a The oligonucleotides contain the letters: F – forward or R – reverse