Table 1 Oligonucleotides used in this study
Primersa | Sequences (5'-3') |
|---|---|
NdeI Ecotin F | GATATACATATGAAGACCATTCTACCTGCAGTA |
Ecotin-Strep SalI R | TATAGTCGACTTATTTTTCGAACTGCGGGTGGCTCCAGCGAACTACCGCGTTGTCAAT |
Ecotin-Ubi (AS2–7) R | CAGGGTCTTCACGAAGATCTGGCGAACTACCGCGTTGTCAAT |
Ubi (AS2–7) F | CAGATCTTCGTGAAGACCCTGACC |
Ubi-Strep XhoI R | TATACTCGAGTTATTTTTCGAACTGCGGGTGGCTCCAGCCACCCCTCAGACGGAGGAC |
lacUV5p SphI F | CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTT |
lacUV5p XbaI R | CTAGAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGCATG |
Ubi-FLS XhoI 1 F | TATGAGTCGACCCTGCACCTGGTCCTCCGTCTCCGCGGTG |
Ubi-FLS XhoI 2 R | CCGCCACCTTTATCGTCATCATCTTTATAGTCACCACCGCGGAGACGGAG |
Ubi-FLS XhoI 3 F | ATGACGATAAAGGTGGCGGAGGTAGCGGTGGAGGAGGCAGCTGGAGCCAT |
Ubi-FLS XhoI 4 R | TATACTCGAGTTATTTTTCAAACTGCGGATGGCTCCAGCTGCCTC |