Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

Figure 4

Purification of Ecotin-Ubiquitin-FLS from periplasmic concentrate by Strep-Tactin® affinity chromatography and follow-up cleavage with UCH-L3. PerC: concentrated periplasmic solution, pellet (P) and supernatant (S). Fractions of the corresponding purification steps are numbered. Fl: flow-through, Wa: wash, El: elution, *: digestion of concentrated eluate (El 1–6) with UCH-L3 for 13 h. The upper lane represents intact recombinant protein. The cleavage product Ecotin-Ubiquitin is labeled with an asterisk.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us