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Figure 2 | Microbial Cell Factories

Figure 2

From: A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

Figure 2

Cellular localization of Ecotin-Ubiquitin-Strep depending on different expression conditions. Expression and secretion of fusion proteins was analyzed by SDS-PAGE. Western blots with antibodies to the periplasmic MBP and cytoplasmic GroEL marker proteins assessed proper cell fractionations. A) Cultivation at 37°C with T7 promoter, B) lacUV5p-dependant expression at 24°C. M: protein marker, -I and +I: before and after induction of expression, IB: inclusion bodies, Cyt: cytoplasm, Per: periplasm, CS: culture supernatant. The arrow indicates secreted fusion protein (25.55 kDa).

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Microbial Cell Factories

ISSN: 1475-2859

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