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Figure 1 | Microbial Cell Factories

Figure 1

From: A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

Figure 1

Schematic representation of the vector used for Ecotin-Ubiquitin-FLS expression. Amino acids of the corresponding genes are numbered. Full-length ecotin with the intrinsic signal sequence (1–20) was cloned upstream of mouse ubiquitin B (ubi, 2–76), followed by the FLS (1–26) target peptide. The cleavage site for a deubiquitinating enzyme (DUB) is indicated with an arrow. A SacII cleavage was introduced at the ubiquitin C-terminus by PCR. t0, transcription termination region.

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Microbial Cell Factories

ISSN: 1475-2859

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