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Figure 7 | Microbial Cell Factories

Figure 7

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 7

Protein expression as function of cell dry weight - characteristics of different expression systems. Direct correlation of the protein expression via GFP fluorescence to the biomass concentration of the fermentations of figures 5 and 6; calibrated data from measurements in the BioLector: scattered light (ex: 620 nm/em: -, gain: 20), GFP (ex: 485 nm/em: 520 nm, gain: 10) and in the fermenter bypass: OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm).

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