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Figure 6 | Microbial Cell Factories

Figure 6

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 6

Comparison of parallel H. polymorpha fermentations in microtiter plate and stirred tank fermenter. Operation conditions: MTP: 200 μL filling volume, at 30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, gain: 20) and GFP (ex: 485 nm/em: 520 nm, gain: 10); fermenter: 1.4 L filling volume, at 30°C temperature, 800 rpm shaking frequency, 1 vvm specific aeration rate, OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm).

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