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Figure 5 | Microbial Cell Factories

Figure 5

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 5

Comparison of parallel E. coli fermentations in microtiter plate and stirred tank fermenter. Operation conditions: MTP: 200 μL filling volume, at 32°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, gain: 20) and GFP (ex: 485 nm/em: 520 nm, gain: 10); fermenter: 1.4 L filling volume, at 32°C temperature, 1000 rpm shaking frequency, 1 vvm specific aeration rate, induction with 0.5 mM IPTG (end concentration in the fermenter) at 7.3 h, OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm)

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Microbial Cell Factories

ISSN: 1475-2859

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