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Figure 2 | Microbial Cell Factories

Figure 2

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 2

Calibration of measurement signals. (A) Cell dry weight versus scattered light intensities measured in microtiter plates; (B) Cell dry weight versus OD measured in the fermenter bypass; (C) GFP fluorescence in the microtiter plate versus GFP fluorescence in the fermenter of the H. polymorpha fermentation; Operation conditions: MTP (H. polymorpha/E. coli): 200 μL filling volume, at 30°C/32°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, gain: 20) and GFP (ex: 485 nm/em: 520 nm, gain: 10); fermenter (H. polymorpha/E. coli): 1.4 L filling volume, at 30°C/32°C temperature, 800/1000 rpm shaking frequency, 1 vvm specific aeration rate, OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm).

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