Figure 4
From: An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
Growth curves of E. coli BL21(DE3) cells containing BL21(DE3)/pET30a- glf(2-RD5) - xynB , BL21(DE3)/pET30a- glf - xynB and BL21(DE3)/pET30a. (A) in M9 minimal medium supplemented with 20 g L-1 xylose. (B) in M9 minimal medium supplemented with 10 g L-1glucose and 10 g L-1 xylose. (C) in M9 minimal medium supplemented with 20 g L-1 xylose, adding 5 g L-1 glucose after 1 h IPTG induction. E. coli BL21(DE3)/pET30a (filled black square), wild type E. coli BL21(DE3)/pET30a-glf-xynB (filled black circle) and E. coli BL21(DE3)/pET30a-glf(2-RD5)-xynB (filled black triangle).