Xylose inhibition assay for Glf mutant 2-RD5. (A) Xylose inhibition of pNPX transport (normalized by dry cell weight) for BL21(DE3)/pET30a-glf(2-RD5)-xynB (gray columns) and BL21(DE3)/pET30a-glf-xynB (black columns). The inhibition assay was done in the presence of 2% glucose. (B) Relationships between the invert of the initial velocity of pNPX transport and the concentrations of xylose for BL21(DE3)/pET30a-glf(2-RD5)-xynB. All data represent triplicate measurements of same cultures.