Figure 2
From: An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
Schematic overview of random deletion used in this study. A) Linearization of the plasmid by restriction digestion at Sca I site. B) Random deletion with Exo III. C) Blunt-ended with mung bean nuclease and Klenow fragment. D) Single digested with Bgl II. E) Random ligation with T4 DNA ligase.