Figure 2From: An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coliSchematic overview of random deletion used in this study. A) Linearization of the plasmid by restriction digestion at Sca I site. B) Random deletion with Exo III. C) Blunt-ended with mung bean nuclease and Klenow fragment. D) Single digested with Bgl II. E) Random ligation with T4 DNA ligase.Back to article page