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Table 7 Benchmarking of properties relevant for selection of microbial production hosts

From: Microbial production host selection for converting second-generation feedstocks into bioproducts

Scoring Performance parameter S. cerevisiae P. stipitis A. niger T. reesei E. coli C. glutamicum
0, 1 Vitamin requirement 0 0 1 1 1 0
0, 1, 2 Growth on xylose 0 2 2 2 2 0
0, 1, 2 Growth on glucose 2 2 2 2 2 1
0, 1, 2 Growth on arabinose 0 0 2 0 2 0
0, 1, 2 Growth on galactose 1 2 0 0 2 1
0, 1, 2 Growth on mannose 2 2 2 2 2 1
0, 1, 2 Growth on glycerol 1 2 2 0 2 1
TOTAL medium versatility 6 10 11 7 13 4
C-source uptake rate        
0, 1,2,3,4 * Glucose 4 2 1 1 4 3
  Xylose 0 4 1 1 4 0
  Glycerol 1 2 1 0 3 3
Biomass yield        
0, 1,2,3,4 ** Glucose 1 1 3 2 2 3
  Xylose 0 1 3 2 2 0
  Glycerol 4 1 2 0 3 1
TOTAL fermentation parameters 10 11 11 6 18 10
0,1,2 pH <4 1 1 2 2 0 0
0,1,2,3,4 acetic acid resistance 2 2 4 4 2 3
0,1,2,3,4 HMF resistance 2 3 4 4 3 3
0,1,2,3,4 furfural resistance 2 2 2 2 2 1
0,1,2,3,4 NaCl resistance 1 2 4 4 2 2
0,1,2 MgSO4 resistance 2 2 2 2 1 2
TOTAL inhibitors 10 12 18 18 10 11
C-source utilization        
0, 1,2 *** Sugar cane bagasse (EH) 2 2 1 1 2 2
  Sugar cane bagasse (AH) 2 2 1 1 2 2
  Wheat straw (EH) 2 2 1 1 2 2
  Wheat straw (AH) 2 2 1 1 2 2
  Corn stover (AH) 2 2 1 1 2 2
  Willow wood (AH) 2 2 1 1 0 0
  Glycerol from Biodiesel 0 2 1 1 0 0
Biomass concentration        
0, 1,2,3,4 **** Sugar cane bagasse (EH) 1 4 2 2 3 4
  Sugar cane bagasse (AH) 2 2 3 3 1 2
  Wheat straw (EH) 2 3 3 2 1 2
  Wheat straw (AH) 2 3 3 3 3 2
  Corn stover (AH) 2 3 3 3 2 2
  Willow wood (AH) 2 3 3 3 0 0
  Glycerol from Biodiesel 0 2 3 3 0 0
TOTAL feedstock versatility 23 34 27 26 20 22
TOTAL score 49 67 67 57 61 47
  1. 0 = no growth, 1-4 or 1-2 = low to high score on performance parameters indicated.
  2. * C-source uptake rate [g/g/h]: no growth = 0, <0.2 = 1, <0.6 = 2, <1.5 = 3, >1.5 = 4
  3. ** Biomass yield [g/g]: no growth = 0, <0.15 = 1, <0.3 = 2, <0.45 = 3; >4.5 = 4
  4. *** C-source utilization, time [h] after which > 50% of the initial glucose/glycerol was consumed: no growth = 0, >70 = 1, >30 = 2
  5. **** Biomass concentration [g/l]: <0.5 = 0, <2.0 = 1, <5.0 = 2, <10.0 = 3, 10.0 = 4