Skip to main content

Table 7 Benchmarking of properties relevant for selection of microbial production hosts

From: Microbial production host selection for converting second-generation feedstocks into bioproducts

Scoring

Performance parameter

S. cerevisiae

P. stipitis

A. niger

T. reesei

E. coli

C. glutamicum

0, 1

Vitamin requirement

0

0

1

1

1

0

0, 1, 2

Growth on xylose

0

2

2

2

2

0

0, 1, 2

Growth on glucose

2

2

2

2

2

1

0, 1, 2

Growth on arabinose

0

0

2

0

2

0

0, 1, 2

Growth on galactose

1

2

0

0

2

1

0, 1, 2

Growth on mannose

2

2

2

2

2

1

0, 1, 2

Growth on glycerol

1

2

2

0

2

1

TOTAL medium versatility

6

10

11

7

13

4

C-source uptake rate

       

0, 1,2,3,4 *

Glucose

4

2

1

1

4

3

 

Xylose

0

4

1

1

4

0

 

Glycerol

1

2

1

0

3

3

Biomass yield

       

0, 1,2,3,4 **

Glucose

1

1

3

2

2

3

 

Xylose

0

1

3

2

2

0

 

Glycerol

4

1

2

0

3

1

TOTAL fermentation parameters

10

11

11

6

18

10

0,1,2

pH <4

1

1

2

2

0

0

0,1,2,3,4

acetic acid resistance

2

2

4

4

2

3

0,1,2,3,4

HMF resistance

2

3

4

4

3

3

0,1,2,3,4

furfural resistance

2

2

2

2

2

1

0,1,2,3,4

NaCl resistance

1

2

4

4

2

2

0,1,2

MgSO4 resistance

2

2

2

2

1

2

TOTAL inhibitors

10

12

18

18

10

11

C-source utilization

       

0, 1,2 ***

Sugar cane bagasse (EH)

2

2

1

1

2

2

 

Sugar cane bagasse (AH)

2

2

1

1

2

2

 

Wheat straw (EH)

2

2

1

1

2

2

 

Wheat straw (AH)

2

2

1

1

2

2

 

Corn stover (AH)

2

2

1

1

2

2

 

Willow wood (AH)

2

2

1

1

0

0

 

Glycerol from Biodiesel

0

2

1

1

0

0

Biomass concentration

       

0, 1,2,3,4 ****

Sugar cane bagasse (EH)

1

4

2

2

3

4

 

Sugar cane bagasse (AH)

2

2

3

3

1

2

 

Wheat straw (EH)

2

3

3

2

1

2

 

Wheat straw (AH)

2

3

3

3

3

2

 

Corn stover (AH)

2

3

3

3

2

2

 

Willow wood (AH)

2

3

3

3

0

0

 

Glycerol from Biodiesel

0

2

3

3

0

0

TOTAL feedstock versatility

23

34

27

26

20

22

TOTAL score

49

67

67

57

61

47

  1. 0 = no growth, 1-4 or 1-2 = low to high score on performance parameters indicated.
  2. * C-source uptake rate [g/g/h]: no growth = 0, <0.2 = 1, <0.6 = 2, <1.5 = 3, >1.5 = 4
  3. ** Biomass yield [g/g]: no growth = 0, <0.15 = 1, <0.3 = 2, <0.45 = 3; >4.5 = 4
  4. *** C-source utilization, time [h] after which > 50% of the initial glucose/glycerol was consumed: no growth = 0, >70 = 1, >30 = 2
  5. **** Biomass concentration [g/l]: <0.5 = 0, <2.0 = 1, <5.0 = 2, <10.0 = 3, 10.0 = 4
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us