Figure 2From: Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus β-1,3-glucanaseBinding of the F5/8C module of LamA to insoluble polysaccharides. The purified protein (20 μg/mL) and the indicate substrate (25 mg/mL) were thoroughly mixed at 37°C for 1 h. The amount of protein remaining in the supernatant (S) and co-precipitating with the substrate (P) were examined by SDS-PAGE.Back to article page